To B12 or not to B12?

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B12 is a hot topic in nutrition, especially amongst vegetarians and vegans. As Live Blood Analysts we spend a lot of time looking at red blood cells through the microscope and one of the most common abnormalities that we see are related to issues with B12.  

Live blood analysts are able to view a drop of blood in its ‘live’ state, meaning it is unstained, has had no chemicals added to it and is fresh, not clotted. Live blood analysis (LBA) is used to assess the state of health of red blood cells, white blood cells, platelets and the contents of the plasma. B12 is vital for normal red blood cell production and a lack of it is obvious when looking at a blood sample. B12 deficiency, if present, will be apparent in the size and shape of the red blood cells and in extreme deficiency we will see certain abnormalities in some of the white blood cells too. The picture below is of a blood sample, magnified 1000x through a darkfield microscope; the cell that has been highlighted is a ‘macrocyte’. A macrocyte is an abnormally large red blood cell and it is released into the blood stream when there is a lack of B12 (and folate, but more about that later). If there are a large proportion of these throughout the blood sample, it is a sign that there may be a B12 deficiency.

 Macrocyte red blood cell

Macrocyte red blood cell

Other cells that indicate issues with B12 are ovalocytes, microcytes and hypersegmented neutrophils.

 ovalocyte red blood cell
 microcyte red blood cell
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LBA is not a diagnostic test but it is a tool that gives a holistic health practitioner an insight into the cellular life of a client through viewing their blood. As B12 is vital for the red blood cells especially, this makes live blood analysis a very useful tool for screening for B12 deficiencies. A skilled practitioner will be able to analyse the blood sample, cross reference it with the client’s case history and not only assess the extent of a B12 issue but also what may be the root cause:

  • Correlations with diet – is there enough B12 available in the food the client eats?

  • Correlations with digestive capacity – is the client suffering from digestive issues that can interfere with digestion and absorption of nutrients?

  • Correlations with other issues found in the blood – is the blood showing a lack of iron? / is the plasma crowded with fibrin, indicating liver issues? / are there signs of leaky gut, indicating dysbiosis? / are the white blood cells out of range, indicating autoimmune issues? / is there a high level of oxidative stress or inflammation?

Even consuming B12 in one’s diet or taking B12 supplements does not always ensure sufficient levels of available B12 for our body to use. How can this be? The answer lies in the complexities of the vitamin B12 itself and the complexities of the human organism.


What is B12 and why is it important?

 B12 chemical formula

Vitamin B12 is the largest, most complicated of all the vitamins and it is also one of the most important nutrients for our health. In our body B12 is used as a co-enzyme that is involved in three vital processes that happen within every single cell of our body. It plays a vital role in the synthesis of DNA, the development of red blood cells and the creation of the myelin sheath that coats the nerve cells that allow the conduction of nervous signals. This is why the effects of a B12 deficiency can cause many profound and varied health issues.

A mild deficiency of B12 may not cause any symptoms as the body keeps a store of it in the liver, but if the root-cause of a deficiency is not corrected it can lead to quite severe and far ranging health issues:

Signs of B12 Deficiency  

Poor growth/failure to thrive in infants                                                 
Inflamed tongue
Premature grey hair                                                                                
Disturbed carbohydrate metabolism
Fatigue or Weakness                                                                                                 
Weight loss                                                                                              
Constipation
Infertility                                                                                                   
Vision problems
Loss of hearing and tinnitus                                                                    
Numbness and tingling in the hands and feet
Alcoholism                                                                                                
Impotence
Incontinence                                                                                             
Hyperpigmentation and hypopigmentation (dark and light patches in the skin)
Neuralgia, neuritis and bursitis                                                                  
Anaemia, including pernicious anaemia                                                
Spinal cord degeneration
Psoriasis and other skin problems                                                           
Brain degeneration
Insomnia                                                                                                   
Irrational or chronic anger                       
Violent behaviour                                                                                    
Lack of balance/abnormal gait                                   
Many emotional disorders

The possible dangers of supplementing folate when B12 deficient

Folate is the name given to the naturally occurring vitamin B9, also known as folic acid when it is in its synthesised form. Both B12 and B9 are coenzymes involved in methylation reactions that allow for DNA synthesis. In a state of B12 deficiency folate (B9) becomes trapped as methylfolate and cannot be further involved in essential reactions for DNA replication. In this situation, if folic acid is given either as supplement or as a fortified food (such as cereals and bread products) the trapped folate is by-passed which temporarily masks the underlying B12 deficiency. The danger in this is that the damage from B12 deficiency continues and eventually symptoms of cognitive decline and anaemia occur. Therefore it is possible that, for those of us that are B12 deficient, it is dangerous to supplement with folic acid and it would be wise to be mindful of how many folic acid fortified foods are consumed.

This makes it crucial for us to determine if we are or at risk of being B12 deficient.

Protective effects against homocysteine

Homocysteine is produced in the body from the metabolism of an amino acid methionine which is ingested as a component of food protein. Homocysteine needs to be converted to S-adenosylmethionine (SAM) so that our body can create glutathione; the body’s most powerful anti-oxidant protecting our cells from free radical damage. If we are deficient in B12 and folate this conversion cannot take place, leaving high levels of homocysteine circulating in our bodies. This can lead to cardiovascular disease and cognitive decline.

Making sure we have good levels of available B12 can protect us from the damage homocysteine can do.


Causes of B12 deficiency

There are a many different possible causes for a lack of ‘usable’ B12 in our systems:

  • Any kind of dysfunction, irritation, inflammation or disease of the digestive tract that diminishes levels of stomach acid or ‘intrinsic factor’ (see later), and limits the transport of B12 out of the digestive tract and into the blood e.g:

Gastritis
Crohn’s disease
Chronic enteritis
Chronic diarrhoea
Pancreatic disease

  • Consumption of alcohol, recreational drugs, toxins, chemical pollutants, or heavy metals can use up stores of B12 and affect production of intrinsic factor and absorption in intestines.

  • Intestinal worms and parasites consume large amounts of B12

  • Aging – our ability to produce stomach acid and intrinsic factor diminishes as we age. Many symptoms of old age are due to a lack of B12.

  • Prescription drugs such as contraception, hormones, diabetes medication, proton pump inhibitors, blood pressure medications, beta blockers, statins and many more block the absorption of B12.

  • Any form of liver congestion, disease or stress limits B12 storage and metabolism.

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The difficulties of effectively testing B12 levels


Serum B12 tests can be inaccurate, leading to false positives and false negatives in mild to severe B12 deficiency:

Testing for B12 deficiency is tricky. The main method for testing is a serum blood test. This assesses levels of B12 in a sample of blood and is the standard test for general medical practice. However it is an unreliable indicator of B12 availability because B12 has a usable and unusable form which a serum test cannot differentiate between. Serum tests results can show a high level of B12, but not whether cells can actually access it.

Testing for the active form of B12 could prove more useful:

The majority of plasma B12 (70 - 90%) is in the form of haptocorrin (HC) and the rest is in the form of transcobalamin (TC), which is the only form that can enter cells. A test for transcobalamin would be more useful in diagnosing B12 deficiency.

Can a urine test offer any help?:

Methylmalonic acid (MMA) is a by-product of amino acid metabolism and is produced when protein is digested. In cases of B12 deficiency, our bodies produce more MMA and it is excreted via the kidneys. High levels of MMA in the urine can be good indication of B12 deficiency.

  Can Live Blood Analysis assess B12 deficiency?:

By directly observing a blood sample magnified 1000x you can assess it for specific cell pathologies that indicate a lack of B12 - specifically macrocytes, microcytes, ovalocytes and hypersegmented white blood cells. If a large percentage of the sample contains these types of cells it is a sure sign of B12 absorption issues. The benefit of this blood analysis is that it can also offer indications as to the root cause of a B12 deficiency.


Can we get enough B12 from our diet?

B12, also known as cobalamin, is produced in animals by their intestinal bacteria. The B12 is then stored in their liver, muscles, eggs and milk. Our digestive tract is designed to absorb this B12 by consuming these animal products. B12 is also produced by anaerobic bacteria present in soils and so can be found on the unwashed skin of plants that are grown in naturally maintained, healthy soils.

 B12 rich foods such as meat, cheese, eggs, and nuts

B12 rich foods such as meat, cheese, eggs, and nuts

Our diet can contain plenty of foods rich in B12 but if our digestive tract is not functioning optimally we may not be able to absorb that B12. We have a very complex digestive chain of reactions that are designed to absorb this vital nutrient, but this chain can be disrupted at many points:

B12 Pathway.jpg

How ‘B12 Analogues’ can block the absorption of real B12

There are different forms that B12 can take but there are only two that can actually be used directly by the body; methylcobalamin and adenosylcobalamin.  There are also two other forms, cyanocobalamin and hyroxocobalamin, that can be converted by our body into the useable forms. Then there are the analogues of B12 that have a similar structure but do not perform any of B12’s biochemical functions in our body. These analogues block the true B12 from being absorbed and so the more of these we consume, the harder it is for us to get enough true B12.

Fresh algae produce true B12 but after drying it only contains the analogue B12. Additionally, the level of B12 available in plant sources can vary as levels depend more on the microorganisms in the environment than the plant. This is one reason why some research on Chlorella shows high levels of true B12 and in other studies it shows there is none. Most plant sources of B12 contain higher levels of analogue B12 than true B12, making them poor sources of B12.


Supplementing B12 effectively

 b12 tincture supplement

Supplementation is often necessary but it can be very confusing as there are so many different types of B12 supplement available:

  • Choose a form of B12 that is most usable by the body – methylcobalamin or adenosylcobalamin. Avoid the cyanocobalamin form as it contains cyanide which, although is in very small amounts, still needs to be processed by the body.

  • With all forms of supplemental B12 check the ingredients for sweeteners, fillers and other additives. It is always best to choose one that is pure or has as few ingredients as possible.

  • B12 supplements come in four types - sublingual, transdermal, intramuscular and capsules/tablets:

Sublingual B12 can be in liquid or tablet formulation. It is deposited under the tongue and absorbed through the mucous membrane of the mouth. Although only 1 to 2% is absorbed this way, if any of the dosage is swallowed it can be absorbed through the digestive tract. Sublingual B12 is useful if the digestive tract is not in optimum health or if there is difficulty in swallowing.

Transdermal B12 is a liquid B12 that is mixed with a carrier molecule that allows the B12 to pass through the skin. This is another useful method of delivery of B12 as it bypasses the digestion. About 6% of the B12 is absorbed this way, but it is important to make sure the skin is free from chemicals as these will get absorbed as well.

Intramuscular B12 is an injection of a B12 liquid into the muscles where is it absorbed into the tissues. The advantages of this form of B12 are that it gets into the blood stream quickly and is therefore useful for those with severe deficiencies. It is also pure, containing very little other than B12 and 70% of it is absorbed, higher than any other form. Only a few injections are required to raise levels and stores of B12, and then a maintenance dose is required every 3 to 6 months, depending on the reasons for deficiency. Note that there are risks of bruising or blisters at the injection site, and also possible infections.

Tablets or capsules of B12 that are swallowed are possibly the least likely to be absorbed as nearly all issues with B12 deficiency are related to digestive issues.

 

Conclusion

Vitamin B12 is essential to our health, yet in order for us to be able to absorb it we need to have a healthy functioning digestive system and the correct type of B12 in our diets. Live Blood Analysis can screen the blood for signs of B12 deficiency and it can also assess other issues that may be contributing to our inability to assimilate B12, such as digestion, absorption and detoxification. Uncovering the root cause of any B12 deficiency issue is the key to knowing what to do so the body can correct itself. The blood can then be reassessed by live blood analysis after a period of three months, by which time the blood cells will have been replaced. If changes to diet and a supplement plan have been implemented then there will be a clear difference in the condition of the blood cells – there will be less macrocytes, microcytes, ovalocytes or hypersegmented neutrophils and more normal healthy red and white blood cells.